effects of phenylglyoxal and n-ethylmaleimide concentration on mycophenolic acid production by penicillium brevi-compactum atcc16024

mycophenolic acid is a secondary extracellular metabolite of penicillium strains with numerous pharmaceutical properties such as antibiotic and immunosuppressive uses. the aim of this work is the survey of the effect of phenylglyoxal and n-ethylmaleimide concentration in culture medium on mycophenolic acid production by penicillium brevi-compactum atcc16024 was investigated. batch submerged fermentation was performed in 250 ml shake flasks at 24°c and 200 rpm in a rotary shaker for 300 h using a basic culture medium containing different concentrations of phenylglyoxal and n-ethylmaleimide ranged from 0 to 20 mg. l-1. for the basic medium without any amounts of phenylglyoxal and n-ethylmaleimide (control), maximum mpa production, product yield and productivity of process was in order, 1.5042 g. l-1, 20.3 mg. g-1 consumed glucose and 5.37 mg. l-1h-1. maximum produced mpa of 2.9032 g. l-1, mpa yield of 39.23 mg. g-1 of consumed glucose, productivity of 10.37 mg. l-1 h-1 and total enhancement of 93.11% was obtained when the culture medium was contained 18 mg. l-1 of phenylglyoxal, represented more than 93% raising in compare to control. maximum mpa concentration, yield and productivity in order was obtained 3.1123 g. l-1, 42.06 mg. g-1 of consumed glucose and 11.11 mg. l-1 h-1, with using 6 mg. l-1 of n-ethylmaleimide. n-ethylmaleimide was caused to 2.138 folds (106.89%) increase in mpa production by p. brevi-compactum atcc16024.